一、基本情况
杨亚军，博士，副研究员
电子邮件：yang_yj@hubu.edu.cn
研究领域：1. 使用单分子磁镊技术揭示RNA、DNA、蛋白质等生物大分子的动态结构及其相互作用，探索生物大分子的结构动态和功能机制；2. 单分子视角下研究病毒基因组的包装、转录和复制；3. 发展单分子力学探针，以应用于单分子检测和机械约束下分子互作的定量研究。
研究团队：william威廉官网病毒学和疫苗研究中心，陈明周教授课题组，链接： https:/info/2059/16972.htm
二、教育背景
2015.09-2020.06 武汉大学，生物物理学，博士
2010.09-2014.06 william威廉官网，生物科学类基地班，学士
三、工作经历
2025.06-至今 william威廉官网，william威廉，副研究员
2020.07-2025.05 武汉大学，william威廉，弘毅博士后/助理研究员
四、科研项目
1. 国家自然科学基金青年项目（32100991）：一种单分子核酸检测技术，2022.01-2024.12，30万元，主持
2. 中国博士后科学基金特别资助（2024T170688）：一种基于单分子操纵的生物分子相互作用定量分析平台，2024.07-2025.05，18万，主持
3. 中国博士后科学基金面上项目（2022M722472）：基于单分子操纵的无扩增核酸检测技术及其在MicroRNAs检测中的应用，2022.11-2024.12，8万元，主持
4. 中央高校基本科研业务费专项（2042021kf0048）：单碱基精度核酸检测条形码自动化技术，2021.01-2022.12，8万元，主持
5. 湖北省科技厅专项（2024AFE008）：基于近红外磁镊的高通量核酸分型定量技术的开发与应用，2024.12-2026.12，100万，骨干
五、代表性论文（#第一作者; *通讯作者）
1. Xiao-Cong Zhao#, Bin Wu#, Ya-Jun Yang#, Ying Li#, Qi-Yuan Qiu#, Liu Wang, Ya-Peng Xu, Han Gong, Lun Song, Xue-Jie Wang, Jing-Yao Shi, Xue-Feng Chen, Shao-Ran Zhang, Qi Zong, Liang Dai, Shi-Shen Du*, Yan Zhang*, Wen-Qiang Wu*, Xing-Hua Zhang*. “N6-methyladenine modification of DNA enhances RecA-mediated homologous recombination”, Proc Natl Acad Sci U S A, 122 (34):e2508652 (2025)；
2. Ya-Jun Yang, Hang Fu, Xiao-Lu Li, Hong-Yu Yang, Er-Chi Zhou, Cheng-Yu Xie, Shu-Wen Wu, Fan He*, Yan Zhang*, Xing-Hua Zhang*. “A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes”, Nucleic Acids Res, 51(17):e90 (2023);
3. Ya-Jun Yang, Hai-Long Dong, Xiao-Wei Qiang, Hang Fu, Er-Chi Zhou, Chen Zhang, Lei Yin, Xue-Feng Chen, Fu-Chao Jia, Liang Dai, Zhi-Jie Tan, Xing-Hua Zhang*. “Cytosine methylation enhances DNA condensation revealed by equilibrium measurements using magnetic tweezers”, J Am Chem Soc, 142 (20):9203-9209 (2020);
4. Ya-Jun Yang, Lun Song, Xiao-Cong Zhao, Chen Zhang, Wen-Qiang Wu, Hui-Juan You, Hang Fu, Er-Chi Zhou, Xing-Hua Zhang*. “A Universal Assay for Making DNA, RNA, and RNA–DNA Hybrid Configurations for Single-Molecule Manipulation in Two or Three Steps without Ligation”, ACS Synth Biol, 8(7):1663-1672 (2019);
5. Chen Zhang#, Hang Fu#, Ya-Jun Yang#, Er-Chi Zhou, Zhi-Jie Tan, Hui-Juan You, Xing-Hua Zhang*. “The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers”, Biophys J, 116 (2):196-204 (2019) (Selected as the best paper of 2019);
6. Bin Wu#, Fu-Jia Tian*#, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*. “High Bendability of Short RNA-DNA Hybrid Duplex Revealed by Single-Molecule Cyclization and Molecular Dynamics Simulations”, Biomolecules, 15, 724 (2025);
7. Er-Chi Zhou#, Hang Fu#, Hao-Ze Wang, Ya-Jun Yang, Xing-Hua Zhang*. “Converting multiple to single DNA-tethered beads and removing one-end-tethered only DNA in high-throughput stretching”, ACS Sens, 9(11):6292−6298 (2024);
8. Xiao-Wei Qiang#, Chen Zhang#, Hai-Long Dong#, Fu-Jia Tian, Hang Fu, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*, Zhi-Jie Tan*. “Multivalent cations reverse the twist-stretch coupling of RNA”, Phys Rev Lett, 128(10):108103 (2022);
9. Hang Fu#, Chen Zhang#, Xiao-Wei Qiang#, Ya-Jun Yang, Zhi-Jie Tan*, Xing-Hua Zhang*. “Opposite effects of high-valent cations on the elasticities of DNA and RNA duplexes revealed by magnetic tweezers”, Phys Rev Lett, 124(5):058101 (2020);
10. Xiao-Cong Zhao, Hang Fu, Lun Song, Ya-Jun Yang, Er-Chi Zhou, Guang-Xue Liu, Xue-Feng Chen, Zhuo Li, Wen-Qiang Wu, Xing-Hua Zhang*. “S-DNA and RecA/RAD51-Mediated Strand Exchange in Vitro”, Biochemistry, 58(15): 2009-2016 (2019);
11. Yan-Xia Jin, Ya-Jun Yang, Yan-Ting Su, Xiang-Dong Ye, Wei Liu, Qing Yang, Jie Wang, Xiang-Nning Fu, Yong-Sheng Gong, Hui Sun*. “Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer”, Glycoconj J, 36(1):57-68 (2019);
12. Wei Liu, Guang-Hui Han, Ya-Lin Yin, Shuai Jiang, Guo-Jun Yu, Qing Yang, Wen-Hui Yu, Xiang-Dong Ye, Yan-Ting Su, Ya-Jun Yang, Gerald W Hart*, Hui Sun*. “AANL (Agrocybe aegerita lectin 2) is a new facile tool to probe for O-GlcNAcylation”, Glycobiology, 28(6):363-373 (2018).
六、申请与授权专利
1. 张兴华，杨亚军。一种分子检测方法，申请号：202310992413.1
七、荣誉及奖励
1. 武汉大学研究生学术创新二等奖（2020）
2. 博士研究生国家奖学金（2019）
